Biotin streptavidin binding sds sheets

Native streptavidin of relatively high mass (66-75 kDa) can be converted to a lower-mass form (i.e., 60 kDa) by proteolytic digestion at both the N and C termini. The lower MW streptavidin is called “core” or “truncated” streptavidin and has better biotin-binding characteristics than the native protein.
When the total biotin binding capacity of the coated surfaces were measured using the DELFIA ® enhancement, the batch of SH–SAv produced with 5-fold molar excess of SATA provided already up to 66% increase in the total biotin binding capacity, compared to the reference surface of native streptavidin (data not shown). With 40-fold molar ... Four of these tryptophan residues are clustered in the hydrophobic binding site in close contact with biotin. The binding pocket is located in a shared interface contact domain, since one of the tryptophans, residue 120, belongs to the adjacent subunit (5 17 20). As expected, biotin binding leads to considerable changes in STV fluorescence.

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Streptavidin Magnetic Beads are 1 μm superparamagnetic particles covalently coupled to a highly?pure form of streptavidin. The beads can be used?to capture?of biotin labeled substrates including antigens, antibodies and nucleic acids.
Streptavidin homotetramers have a particularly high, non-covalent binding affinity for biotin. When conjugated with fluorochromes, streptavidin has been widely used with biotin-conjugated antibodies and other biotinylated specific-binding molecules (eg, recombinant proteins and lectins) to stain cells and tissues for subsequent multiparameter ... Avidin and other biotin-binding proteins, including Streptavidin and NeutrAvidin protein, have the ability to bind up to four biotin molecules, as shown in the diagram below, making this interaction ideal for both purification and detection strategies. Schematic of the Avidin-biotin interaction.

MSD SULFO-TAG labeled Streptavidin may be used as a secondary detection reagent to generate electrochemiluminescence in MESO SCALE DISCOVERY assays. SULFO-TAG Streptavidin is used to report biotin-labeled molecules. It allows the use of commercially available biotinylated detection antibodies in MSD assays. Biotin is used together with streptavidin in the design of many diagnostic assays. These assays leverage the high stability and specificity of the biotin-streptavidin interaction as a means of increasing analytic sensitivity. Examples of assays that may use biotin-streptavidin chemistry include those that measure cardiac biomarkers and hormones.
Native streptavidin of relatively high mass (66-75 kDa) can be converted to a lower-mass form (i.e., 60 kDa) by proteolytic digestion at both the N and C termini. The lower MW streptavidin is called “core” or “truncated” streptavidin and has better biotin-binding characteristics than the native protein. Streptavidin and its relative avidin are tetrameric proteins with D2 symmetry. Each ~15 KD subunit is primarily organized as an antiparallel beta barrel. Streptavidin finds wide application in biotechnology and biomedical applications owing to its high affinity and binding specificity for the vitamin biotin. Using a biotin/avidin or biotin/streptavidin detection system results in an additional layer of amplification over a directly conjugated secondary antibody. AMCA Streptavidin is produced by conjugating streptavidin with a coumarin fluorescent dye, 7-amino-4-methylcoumarin-3-acetic acid.

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Streptavidin biotin-binding activity and enzyme activity (for AP and HRP conjugates only) are retained after conjugation. Conjugate Method of Production Streptavidin-AP Chemical coupling of the enzyme, calf intestinal alkaline phosphatase (CIAP), to streptavidin (Fernley, 1971) Streptavidin-HRP Chemical coupling of the enzyme, horseradish MSD SULFO-TAG labeled Streptavidin may be used as a secondary detection reagent to generate electrochemiluminescence in MESO SCALE DISCOVERY assays. SULFO-TAG Streptavidin is used to report biotin-labeled molecules. It allows the use of commercially available biotinylated detection antibodies in MSD assays.
Avidin and other biotin-binding proteins, including Streptavidin and NeutrAvidin protein, have the ability to bind up to four biotin molecules, as shown in the diagram below, making this interaction ideal for both purification and detection strategies. Schematic of the Avidin-biotin interaction.