Streptavidin biotin-binding activity and enzyme activity (for AP and HRP conjugates only) are retained after conjugation. Conjugate Method of Production Streptavidin-AP Chemical coupling of the enzyme, calf intestinal alkaline phosphatase (CIAP), to streptavidin (Fernley, 1971) Streptavidin-HRP Chemical coupling of the enzyme, horseradish MSD SULFO-TAG labeled Streptavidin may be used as a secondary detection reagent to generate electrochemiluminescence in MESO SCALE DISCOVERY assays. SULFO-TAG Streptavidin is used to report biotin-labeled molecules. It allows the use of commercially available biotinylated detection antibodies in MSD assays.
Avidin and other biotin-binding proteins, including Streptavidin and NeutrAvidin protein, have the ability to bind up to four biotin molecules, as shown in the diagram below, making this interaction ideal for both purification and detection strategies. Schematic of the Avidin-biotin interaction.